Simultaneous measurement of cellular MTT reduction activity and lactate dehydrogenase release using MTT

Reduction of MTT by glutathione S-transferase.

The reduction of the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to a blue formazan product is widely used for assaying cell survival and proliferation. The reduction reaction is catalyzed by dehydrogenases localized in the mitochondria of viable cells. As part of an analysis of the ability of glutathione S-transferase (GST) enzymes to protect cells from...

Proceedings MTT 2011

There is a certain consensus on some defining features of collocations (they are frequent, recurrent, conventional, institutionalized, etc.), but there is not much agreement on other characteristics, for example whether or not they constitute binary lexical relations, and — crucially— whether they are arbitrary, and must be stipulated, or should instead be deduced or inferred. I will argue that...

Single Top Production and Mtt

Measurement of total lactate dehydrogenase activity.

Lactate dehydrogenase (LD: EC 1.1.1.27) is the most important clinically of several dehydrogenases occurring in human serum. Lactate dehydrogenase is cytoplasmic in its cellular location and in any one tissue is composed of one or two of five possible isoenzymes. While many of its clinical applications involve quantification of one or more specific serum isoenzymes, an estimate of total LD is r...

MTT Camera Ready Color.PDF

An in-depth investigation of a multi-mode reconfigurable aperture controlled by pin diode switches is presented. A method for reducing the coupling across the pin diode switches is explored, yielding a noticeable improvement in the isolation characteristics between ports. Strategic feeding of the patch antenna ports results in significantly improved radiation patterns, making the system suitabl...